Construction of a Kluyveromyces lactis ku80 − Host Strain for Recombinant Protein Production: Extracellular Secretion of Pectin Lyase and a Streptavidin–Pectin Lyase Chimera
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چکیده
منابع مشابه
Screening of Alginate Lyase-Producing Bacteria and Optimization of Media Compositions for Extracellular Alginate Lyase Production
Background: Alginate is a linear polysaccharide consisting of guluronate (polyG) and mannuronate (polyM) subunits. Methods: In the initial screening of alginate-degrading bacteria from soil, 10 isolates were able to grow on minimal medium containing alginate. The optimization of cell growth and alginate lyase (algL) production was carried out by the addition of 0.8% alginate and 0.2-0.3 M NaCl ...
متن کاملscreening of alginate lyase-producing bacteria and optimization of media compositions for extracellular alginate lyase production
background: alginate is a linear polysaccharide consisting of guluronate (polyg) and mannuronate (polym) subunits. methods: in the initial screening of alginate-degrading bacteria from soil, 10 isolates were able to grow on minimal medium containing alginate. the optimization of cell growth and alginate lyase (algl) production was carried out by the addition of 0.8% alginate and 0.2-0.3 m nacl ...
متن کاملConstruction of a recombinant wine yeast strain expressing a fungal pectate lyase gene.
A gene fusion between the Saccharomyces cerevisiae actin gene promoter and the cDNA of the Fusarium solani f. sp. pisi pelA gene has been constructed. This expression cassette has been introduced into the industrial wine yeast strain T73. The resulting recombinant strain is able to secrete active PELA enzyme into the culture medium. In preliminary microvinification experiments the wine produced...
متن کاملEnzyme assay for identification of pectin and pectin derivatives, based on recombinant pectate lyase.
A simple method was developed for fast identification of pectin, based on a recombinant endopectate lyase cloned from Aspergillus niger. When pectin was demethylated and treated with pectate lyase, beta-elimination occurred, resulting in a double bond between C-4 and C-5 in the galacturonic acid residue of the released nonreducing end. The formation of double bonds produced an increase in light...
متن کاملA novel, lactase-based selection and strain improvement strategy for recombinant protein expression in Kluyveromyces lactis
BACKGROUND The Crabtree-negative yeast species Kluyveromyces lactis has been established as an attractive microbial expression system for recombinant proteins at industrial scale. Its LAC genes allow for utilization of the inexpensive sugar lactose as a sole source of carbon and energy. Lactose efficiently induces the LAC4 promoter, which can be used to drive regulated expression of heterologou...
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ژورنال
عنوان ژورنال: Molecular Biotechnology
سال: 2014
ISSN: 1073-6085,1559-0305
DOI: 10.1007/s12033-013-9711-3